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BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
Brand: BD 554722
Description
Recommended Assay Procedure:
For the stimulation of cells, various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28).

Specifications
Can be used for the simultaneous fixation and permeabilization of cells prior to intracellular Cyto kine staining | |
Store undiluted at 4°C | |
125 mL |
7.2 | |
Fixation and permeabilization solution | |
Liquid |
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Safety and Handling
- BD Cytofix/Cytoperm Fixation and Permeabilization Solution
Signal Word
- Danger
Hazard Category
- Skin sensitiser Category 1
- Germ cell mutagenicity Category 2
- Carcinogenicity Category 1B
Hazard Statement
- H317-May cause an allergic skin reaction.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P501-Dispose of contents/container to an appropriate treatment and disposal facility in accordancewith applicable laws and regulations, a.
Supplemental information
- MIXTURE LIST-Contains : formaldehyd e...%
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