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3,3',5,5'-Tetramethylbenzidine, 98%, Thermo Scientific Chemicals Product Code.: 11441548
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3,3',5,5'-Tetramethylbenzidine, 98%, Thermo Scientific Chemicals

Product Code. 11441548
5 g
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1 g
5 g
25 g
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Product Code. 11441548

Brand: Thermo Scientific Alfa Aesar A13868.06

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Description

3,3',5,5'-Tetramethylbenzidine, 98%, or TMB, CAS # 54827-17-7, is a colorimetric reagent used in laboratory-based substrate detection.

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • 3,3',5,5'-Tetramethylbenzidine, also known as 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl or TMB, is a colorimetric substrate for detection of horseradish peroxidase-labeled probes
  • Oxidation of 3,3',5,5'-Tetramethylbenzidine results in a pale blue end product that can be detected at 370 or 655 nm. Sulfuric acid (H2SO4) is used to stop the oxidation reaction and stabilize the reading, resulting in a yellow color that can be read at 450 nm

Application

  • 3,3',5,5'-Tetramethylbenzidine is used as a chromogenic visualization agent in enzyme-linked immunosorbent assays (ELISA) or immunohistochemistry (IHC) techniques
  • 3,3',5,5'-Tetramethylbenzidine has been used to detect molecules in aqueous samples such as copper ion and hypochlorite
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 54827-17-7
Molecular Formula C16H20N2
Molecular Weight (g/mol) 240.35
MDL Number MFCD00007748
InChI Key UAIUNKRWKOVEES-UHFFFAOYSA-N
Synonym 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insolubleShow MoreShow Less
PubChem CID 41206
IUPAC Name 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
SMILES CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Specifications

Melting Point 168°C to 171°C
Quantity 5 g
Beilstein 2808541
Sensitivity Light and moisture sensitive
Solubility Information Soluble in ethyl acetate,ethanol,acetone,chloroform,toluene and dimethyl sulfoxide. Insoluble in water.
Formula Weight 240.35
Percent Purity 98%
Chemical Name or Material 3,3',5,5'-Tetramethylbenzidine

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Lot Number Certificate Type Date Catalog Number
Lot Number10241815 Certificate TypeCertificate of Analysis Date05/07/2022 Catalog NumberA13868.03,A13868.14,A13868.06
Lot NumberI8547A Certificate TypeCertificate of Analysis Date22/11/2021 Catalog NumberA13868.03,A13868.14,A13868.06
Lot NumberX19B034 Certificate TypeCertificate of Analysis Date22/11/2021 Catalog NumberA13868.03,A13868.14,A13868.06
Lot Number10098592 Certificate TypeCertificate of Analysis Date22/11/2021 Catalog NumberA13868.03,A13868.14,A13868.06
Lot NumberG3256A Certificate TypeCertificate of Analysis Date22/11/2021 Catalog NumberA13868.03,A13868.14,A13868.06

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