Cell Function Reagents and Kits

Cell Function Reagents and Kits
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Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Useful with a variety of fluorescence-detection methodologies

Invitrogen™ 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose)
2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability.

Invitrogen™ alamarBlue™ and alamarBlue™ HS Cell Viability Reagents
The alamarBlue cell viability reagents are ready-to-use resazurin-based solutions that function as cell health indicators by using the reducing power of living cells to quantitatively measure viability.

Shipping Condition | Wet Ice |
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Content And Storage | Store in refrigerator at 2°C to 8°C Protect from light. |
Form | Liquid |
Product Type | Cell Viability Reagent |
Emission | 590 nm |
Concentration | 10X |
For Use With (Application) | Viability Assay |
Product Line | alamarBlue™ |
Detection Method | Colorimetric, Fluorescence |
Invitrogen™ Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)
Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential.

Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit
Fluo-4 Direct™ Calcium Assay Kit

Shipping Condition | Room Temperature |
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Modification | Chemical |
Sub Cellular Localization | Nucleus, Organelles, Cytoplasm |
Form | Powder |
For Use With (Equipment) | Confocal Microscope,Fluorescence Microscope,High Content Analysis Instrument,HTS Reader,Microplate Reader,Fluorescent Imager |
Color | Green |
Emission | Visible |
Dye Type | Fluorescent Dye-Based |
Product Line | Molecular Probes™ |
Detection Method | Fluorescence |
Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
Shipping Condition | Room Temperature |
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Format | Tube(s) |
Product Type | Cell Proliferation Kit |
For Use With (Equipment) | Flow Cytometer |
Solubility | DMSO (Dimethylsulfoxide) |
Emission | 405⁄450 |
For Use With (Application) | Proliferation Assay |
Product Line | CellTrace™ |
Detection Method | Fluorescence |
Invitrogen™ Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Sensitive, one-step assay to detect hydrogen peroxide (H2O2) or peroxidase activity
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.

Substrate | HRP (Horseradish Peroxidase) Substrate |
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Shipping Condition | Room Temperature |
Content And Storage | Store in freezer (-5°C to -30°C). |
Form | Solid |
Product Type | Red Hydrogen Peroxide |
For Use With (Equipment) | Fluorometer,Microplate Reader |
Validated Application | Cell Analysis |
For Use With (Application) | Hydrogen Peroxidase Assay |
Product Line | Amplex™ |
Detection Method | Fluorescence |
Molecular Probes™ LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.

Shipping Condition | Room Temperature |
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Description | LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation |
Form | Solid |
Solubility | DMSO (Dimethylsulfoxide) |
Color | Violet |
Emission | 451 nm |
Dye Type | LIVE/DEAD™ Fixable Violet Dead Cell Stain |
For Use With (Application) | Viability Assay |
Excitation Wavelength Range | 416 nm |
Detection Method | Fluorescence |
Cell Type | Eukaryotic Cells |
Cell Permeability | Impermeant |
Format | Tube(s) |
Product Type | Stain |
For Use With (Equipment) | Flow Cytometer |
Product Line | LIVE/DEAD™ |
Molecular Probes™ LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation
The LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.

Shipping Condition | Room Temperature |
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Description | LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation |
Form | Solid |
Solubility | DMSO (Dimethylsulfoxide) |
Color | Blue |
Emission | 450 nm |
Dye Type | LIVE/DEAD™ Fixable Blue Dead Cell Stain |
For Use With (Application) | Viability Assay |
Excitation Wavelength Range | 350 nm |
Detection Method | Fluorescence |
Cell Type | Eukaryotic Cells |
Cell Permeability | Impermeant |
Format | Tube(s) |
Product Type | Dead Cell Stain Kit |
For Use With (Equipment) | Flow Cytometer |
Product Line | LIVE/DEAD™ |
Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red)
A two-color fluorescent cell viability assay that simultaneously identifies live and dead cells. Live cells are labeled with calcein dye and emit a green fluorescence, while dead cells are labeled with SYTOX Deep Red dye and emit a red fluorescence.

Invitrogen™ Image-iT™ Green Hypoxia Reagent
Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells.

Shipping Condition | Approved for shipment on Wet or Dry Ice |
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Content And Storage | Store at -5 to -30°C. Protect from light. |
Product Type | Reagent |
Reagent Type | Hypoxia Reagent |
Product Line | Image-iT™ |
Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

Shipping Condition | Room Temperature |
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Format | Tube(s) |
Product Type | Cell Proliferation Kit |
For Use With (Equipment) | Flow Cytometer |
Emission | 488 nm |
Product Line | CellTrace™ |
Detection Method | Fluorescence |
Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM)
Stain live or fixed cells with this far-red DNA stain.

Content And Storage | Store at 4°C. |
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Purity or Quality Grade | > 99% |
Form | Liquid |
Solubility | Soluble at working concentrations in water and typical buffers |
Color | Red |
Emission | 681 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Cell Imaging |
Excitation Wavelength Range | 647 nm |
Detection Method | Fluorescence |
Cell Permeability | Cell-permeant |
Sub Cellular Localization | Nucleus, Cytosol, Cytoplasm |
Product Type | Fluorescent Probe Solution |
For Use With (Equipment) | Fluorescence Microscope,High Content Analysis Instrument |
Product Line | Pierce™ |
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.

Product Type | Dye |
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For Use With (Equipment) | Flow Cytometer |
Color | Infrared |
Emission | 633/780 |
For Use With (Application) | Viability Assay |
Product Line | eBioscience™ |
Detection Method | Fluorescence |