Life Science Research ELISA Kits

ELISA kits and systems for the quantification of target antigens in human, mouse, rat, bovine, and other tissue extracts, serums, and cell cultures; includes highly validated materials for accurate quantitation and long-term reproducibility.

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Invitrogen™ VEGF Human ELISA Kit

Quantifies natural or recombinant human Vascular Endothelial Growth Factor (VEGF) in serum, plasma, buffered solution, or cell culture medium

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Specifications

Target Species	Human
Conjugate	Biotin
Interassay CV	8.1%
Intraassay CV	4.7%
Incubation Time	4 hr.
Gene Symbol	MVCD1,VEGF,VPF
Total Assay Time	4 hr.
Sample Volume	Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	23.4-1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hr. 20 min.
Accession Number	P15692
Regulatory Status	RUO
Target	Human
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ IL-6 Human Uncoated ELISA Kit

Uncoated ELISA Kit

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Specifications

Target Species	Human
Conjugate	Biotin
Gene Symbol	DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2
Total Assay Time	24 hr. 30 min.
Sample Volume	Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2-200 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr. 45 min.
Accession Number	P05231
Regulatory Status	RUO
Target	Human
Kit Contents	Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme:Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	3569
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-6

Invitrogen™ IL-18 Human ELISA Kit

For the quantitative detection of human IL-18.

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Pricing and Availability
Specifications

Target Species	Human
Conjugate	Biotin
Interassay CV	8.1%
Intraassay CV	6.5%
Gene Symbol	IGIF,IL-18,IL-1g,IL1F4
Total Assay Time	3 hr. 10 min.
Sample Volume	Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Assay Sensitivity	9.0 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	78-5,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr. 20 min.
Accession Number	Q14116
Regulatory Status	RUO
Target	Human
Kit Contents	Pre-coated 96 well plate,Adhesive Plate Covers,Sample Diluent,Assay Buffer concentrate,Assay Buffer concentrate,SAV-HRP,SAV-HRP,Wash Buffer,Chromogen,Chromogen,Stop Solution
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	3606
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-18, Interferon-gamma-inducing factor

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

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Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

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Invitrogen™ IL-6 Human ELISA Kit

Measure levels of human interleukin-6 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

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Specifications

Measurement	Absorbance at 450nm minus absorbance at 550nm
Immunoassay Kit Format	Colorimetric ELISA (TMB substrate) with Plates
Specificity	No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Description	Human IL-6 ELISA Kit
Precision	Intra-assay CV <10%; inter-assay CV <10%
Total Assay Time	3 hr.

Invitrogen™ TNF alpha Human Uncoated ELISA Kit

Uncoated ELISA Kit

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Pricing and Availability
Specifications

Target Species	Human
Conjugate	Biotin
Gene Symbol	CANDF6,IL-17F,ML-1,ML1
Total Assay Time	24 hr. 30 min.
Sample Volume	Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr. 45 min.
Accession Number	P01375
Regulatory Status	RUO
Target	Human
Kit Contents	Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a, tnf-alpha

Invitrogen™ IL-8 Human Uncoated ELISA Kit

Uncoated ELISA Kit

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Pricing and Availability
Specifications

Target Species	Human
Conjugate	Biotin
Gene Symbol	GMCSF
Total Assay Time	24 hr. 30 min.
Sample Volume	Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2-250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr. 45 min.
Accession Number	P10145
Regulatory Status	RUO
Target	Human
Kit Contents	Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.,96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

Invitrogen™ Cortisol Competitive Human ELISA Kit

Competitive ELISA

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Specifications

Target Species	Human
Conjugate	HRP
Description	Cortisol Competitive ELISA Kit
Interassay CV	8.1%
Intraassay CV	8.8%
Total Assay Time	1 hr. 30 min.
Assay Sensitivity	17.3 pg/mL
Storage Requirements	-20°C
Research Discipline	Inflammation
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hr. 20 min.
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Human
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Sample Type	Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ Glucose Colorimetric Detection Kit

Assay Glucose levels in Serum, Plasma, Urine, Buffers and Tissue Culture Media.

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Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Colorimetric Detection Kit
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Product Type	Glucose Colorimetric Detection Kit
Gene ID (Entrez)	Non-Gene
Sample Type	Other Biological Fluids,Plasma,Serum
Gene Symbol	Non-Gene
Target Kit Named	Glucose
Detection Method	Colorimetric

Invitrogen™ PSA (Total)/KLK3 Human ELISA Kit

Sandwich ELISA Kit

Pricing and Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	Biotin
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	KLK3
Total Assay Time	4 hr. 45 min.
Assay Sensitivity	8 pg/mL
Storage Requirements	2°C to 8°C
Research Discipline	Angiogenesis
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr. 20 min.
Assay	Solid-phase sandwich ELISA
Accession Number	P07288
Regulatory Status	RUO
Target	Human
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	354
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	APS, KLK2A1, PSA, hK3

Invitrogen™ Human IL-35 ELISA Kit

For the quantitative detection of Human IL-35

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Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit

Competitive ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Description	Triiodothyronine (T3) Competitive ELISA Kit
Interassay CV	13.4%
Intraassay CV	6.3%
Total Assay Time	2 hr. 30 min.
Assay Sensitivity	37.4 pg/mL
Storage Requirements	-20°C
Research Discipline	Metabolism
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hr. 20 min.
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Chemical
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Sample Type	Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

Competitive ELISA

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Specifications

Target Species	Human
Conjugate	HRP
Description	Thyroxine (T4) Competitive ELISA Kit
Interassay CV	7%
Intraassay CV	3%
Total Assay Time	1 hr. 30 min.
Assay Sensitivity	0.29 ng/mL
Storage Requirements	-20°C
Research Discipline	Metabolism
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hr. 20 min.
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Chemical
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Sample Type	Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ TNF alpha Human ELISA Kit

Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

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Pricing and Availability
Specifications

Target Species	Human
Conjugate	Biotin
Interassay CV	7.5%
Intraassay CV	4.4%
Incubation Time	4 hr.
Gene Symbol	DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Total Assay Time	4 hr.
Sample Volume	Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity	1.7 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.6-1000 pg/mL
Marker Gene	TNF-α
For Use With (Application)	Colorimetric Detection
Test Time	1 hr. 20 min.
Accession Number	P01375
Regulatory Status	RUO
Target	Human
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA Kit
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Life Science Research ELISA Kits

Life Science Research ELISA Kits

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