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Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

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Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

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Pricing and Availability
Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR™
Detection Method	SYBR

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

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Pricing and Availability
Specifications

Standard PCR Reagents and Kits

Content And Storage	Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP
Quantity	1 Kit
For Use With (Application)	Standard PCR

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

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Real Time PCR Assays and Arrays

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

Pricing and Availability
Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	120 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Concentration	50 U/μL
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

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Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	PowerUP™, SYBR™
Detection Method	SYBR

Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Promotions are available

Pricing and Availability
Specifications

Reverse Transcriptases

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ DreamTaq PCR Master Mixes (2X)

Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl_2, and dNTPs.

Pricing and Availability
Specifications

Standard PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
For Use With (Application)	Standard PCR

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

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Pricing and Availability
Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Size (Final Product)	Up to 7 kb
Reaction Format	Premixed Components
Final Product Type	First-Strand cDNA
Reaction Speed	30 to 60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	37°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

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Pricing and Availability
Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase • RiboLock RNase Inhibitor • 5X Reaction Buffer • dNTP Mix • Oligo(dT)₁₈ Primer • Random Hexamer Primer • Control GAPDH RNA • Forward GAPDH Primer (10 μM) • Reverse GAPDH Primer (10 μM) • Nuclease-free water Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
For Use With (Application)	RT-PCR
Format	Kit
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Pricing and Availability
Specifications

Real Time PCR Assays and Arrays

Shipping Condition	Room Temperature
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	60 X
Product Type	Gene Expression Assay
Reaction Speed	Fast
Green Features	Sustainable packaging
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Invitrogen™ SuperScript™ II Reverse Transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.

Promotions are available

Pricing and Availability
Specifications

Reverse Transcriptases

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript II
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ Oligo(dT)₁₈ Primer

Thermo Scientific Oligo(dT)₁₈ Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

Promotions are available

Pricing and Availability
Specifications

Oligonucleotides

Shipping Condition	Dry Ice
Primer Length	18-mer
Form	Liquid
Product Type	Primer
Primer	Oligo dT
Concentration	0.5 μg/μL
For Use With (Application)	cDNA Synthesis

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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)