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Thermo Scientific™ Pierce™ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
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Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
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Form | Powder |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA, Immunohistochemistry |
Product Line | Pierce™ |
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
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Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
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Content And Storage | 2°C to 8°C |
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Sensitivity | ∼1 pg |
Product Type | ECL Western Blot Detection Reagent |
For Use With (Application) | Sensitive Quantitative or Confirmatory Western Blot |
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.
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Content And Storage | 2°C to 8°C |
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Sensitivity | ∼10 pg |
Product Type | ECL Western Blot Detection Reagent |
For Use With (Application) | Western Blot |
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
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Shipping Condition | Room Temperature |
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Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
Conjugate | HRP |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 24 hr. |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
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Substrate | Horseradish Peroxidase |
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Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 8 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
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Substrate | Horseradish Peroxidase |
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Content And Storage | Upon receipt store at 2-8°C. Product is shipped at ambient temperature. |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 1 to 2 hr. |
Product Line | Pierce™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Sensitivity | Low picogram to mid-femtogram |
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Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | Pierce™ |
Detection Method | Chemiluminescence, Fluorescence |
Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution
Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
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Shipping Condition | Wet Ice |
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Substrate | Horseradish Peroxidase |
Sensitivity | Low-femtogram to high-attogram |
Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
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Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Shipping Condition | Dry Ice |
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Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
Product Type | Protein Labeling Reagent |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Membrane Compatibility | PVDF, Nitrocellulose |
Shelf Life | At least 2 years from receipt |
Run Time | 10 minutes |
For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Product Line | Invitrogen™ |
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
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Substrate | Horseradish Peroxidase |
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Sensitivity | Mid-femtogram |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 24 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ DAB Substrate Kit
Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.
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Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
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Substrate | HRP (Horseradish Peroxidase) Substrate |
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Shipping Condition | Room Temperature |
Content And Storage | Store in freezer (-5°C to -30°C). |
Form | Solid |
Product Type | Red Hydrogen Peroxide |
For Use With (Equipment) | Fluorometer,Microplate Reader |
Validated Application | Cell Analysis |
For Use With (Application) | Hydrogen Peroxidase Assay |
Product Line | Amplex™ |
Detection Method | Fluorescence |