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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | MultiScribe |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 120 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | MultiScribe |
Size (Final Product) | Up to 7 kb |
Reaction Format | Premixed Components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 to 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 37°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ SimpliAmp™ Thermal Cycler
The SimpliAmp Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you've come to expect from Applied Biosystem instruments.
Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
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Block Configurations | 96-well, 0.2 mL VeriFlex Block |
User Interface | USB, Wi-Fi, Ethernet |
Wattage | 600 W |
Dimensions | 18.1 x 9.5 x 8.3 in. (46 x 24 x 21 cm) |
Max. Ramp Rate | 4°C/sec. (Block), 3°C/sec. (Sample) |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermal Range | 0°C to 100°C |
Display Type | 8 in. Color TFT LCD Touchscreen |
Product Type | Thermal Cycler |
Capacity | 96-well, 3 Zones VeriFlex |
For Use With (Equipment) | GeneAmp 2700, GeneAmp 9700, Veriti Thermal Cycler, 2720 Thermal Cycler |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
Concentration | 200 U/μL |
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Applied Biosystems™ ProFlex™ PCR System, 3 x 32-well
ProFlex™ PCR Systems are the latest of Applied Biosystems™ thermal cyclers, providing the reliability and performance you've come to expect from Applied Biosystems instruments. By combining an extended warranty with your instrument purchase, you'll extend the life of your investment and receive the support you need to keep your instrument running at peak performance.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
Block Configurations | 3 x 32-well, 2-Zone VeriFlex Blocks |
Memory | USB and On-board |
User Interface | USB, WiFi, Cloud, Ethernet |
Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
Block Format | Interchangeable |
Max. Ramp Rate | 6°C/sec. (Block), 4.4°C/sec. (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | High-throughput Compatible |
Weight | 41 lb. (18.8 kg) |
Thermal Range | 0°C to 100°C |
Display Type | 8.4 in. Color TFT LCD Touchscreen |
Product Type | Thermal Cycler |
Reaction Speed | Fast, Standard |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Applied Biosystems™ Veriti™ Thermal Cycler, 96-well Fast
The Applied Biosystems Veriti Thermal Cycler delivers the proven reliability of Applied Biosystems instruments. With the added control of VeriFlex technology, you have six independent temperature blocks that provide precise control over your PCR optimization. The Veriti 96-Well Fast Thermal Cycler is equipped with a Fast 0.1-mL block configuration.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
Block Configurations | 96-well, 6-Zone VeriFlex Block |
Reaction Volume Range | 10 to 30 μL |
Memory | USB and On-board |
Dimensions | 19.1 x 9.3 x 9.6 in. (48.5 x 23.7 x 24.5 cm) |
Max. Ramp Rate | 5°C/sec. (Block), 4.25°C/sec. (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | High-throughput Compatible |
Weight | 25 lb. (11.4 kg) |
Thermal Range | 0°C to 100°C |
Sample Ramp Rate | ±4.25°C/sec. |
Format | 0.1 mL Tubes, 96-well Plate |
Product Type | Thermal Cycler |
For Use With (Equipment) | Veriti™ Thermal Cycler |
Reaction Speed | Fast, Standard |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Temperature Range (Metric) | 4°C to 99.9°C |
Peak Block Ramp Rate | 5.0°C/sec. |
Product Line | Veriti™ |
Applied Biosystems™ ProFlex™ PCR System, 2 x 384-well
ProFlex™ PCR Systems are the latest in our line of Applied Biosystems™ thermal cyclers, providing the reliability and performance you've come to expect from Applied Biosystems instruments.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
Block Configurations | 2 x 384-well Isothermal Blocks |
Memory | USB and On-board |
User Interface | USB, WiFi, Cloud, Ethernet |
Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
Max. Ramp Rate | 3°C/sec. (Block), 1.6°C/sec. (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | High-throughput Compatible |
Weight | 45 lb. (20.4 kg) |
Thermal Range | 0°C to 100°C |
Display Type | 8.4 in. Color TFT LCD Touchscreen |
Product Type | Thermal Cycler |
Reaction Speed | Fast, Standard |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Product Line | ProFlex™ |
Applied Biosystems™ ProFlex™ PCR System, 2 x 96-well
The ProFlex 2 x 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you've come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
Block Configurations | 2 x 96-well Isothermal Blocks |
Memory | USB and On-board |
User Interface | USB, WiFi, Cloud, Ethernet |
Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
Block Format | Interchangeable |
Max. Ramp Rate | 3°C/sec. (Block), 1.6°C/sec. (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | High-throughput Compatible |
Thermal Range | 0°C to 100°C |
Display Type | 8.4 in. Color TFT LCD Touchscreen |
Product Type | Thermal Cycler |
Reaction Speed | Fast, Standard |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Applied Biosystems™ ProFlex™ PCR System, 96-well
The ProFlex 96-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you've come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
Memory | USB and On-board |
User Interface | USB, WiFi, Cloud, Ethernet |
Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
Block Format | Interchangeable |
Max. Ramp Rate | 6°C/sec. (Block), 4.4°C/sec. (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | High-throughput Compatible |
Weight | 41 lb. (18.8 kg) |
Thermal Range | 0°C to 100°C |
Display Type | 8.4 in. Color TFT LCD Touchscreen |
Product Type | Thermal Cycler |
Reaction Speed | Fast, Standard |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Applied Biosystems™ Veriti™ Thermal Cycler, 60-well
The Applied Biosystems™ Veriti™ 60-Well Thermal Cycler (for 0.5 mL tubes) delivers the proven reliability of Applied Biosystems™ instruments with the 0.5 mL format you need to accommodate larger reaction volumes.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
Tm Calculator | Menu Driven through Touchscreen |
Reaction Volume Range | 25 to 100 μL |
Memory | USB and On-board |
User Interface | USB |
Block Format | Non-interchangeable |
Max. Ramp Rate | 3.3°C/sec. (Block), 2.7°C/sec. (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermal Range | 0°C to 100°C |
Sample Ramp Rate | ± 2.7°C/sec. |
Format | 0.5 mL Tubes |
Display Type | 6.5 in. VGA 32K Color Touchscreen |
Remarks | Easy-to-handle Reaction Tubes for Large Volume Reactions |
Product Type | Thermal Cycler |
Capacity | 1 x 60-well |
Reaction Speed | Standard |
Voltage | 100/240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Temperature Range (Metric) | 4.0°C to 99.9°C |
Peak Block Ramp Rate | 3.3°C/sec. |
Product Line | Veriti™ |
Applied Biosystems™ Veriti™ Thermal Cycler, 384-well
Veriti™ Thermal Cyclers deliver the proven reliability of Applied Biosystems™ instruments with the volume, throughput, block format, and pre-programmed method options to accommodate your research needs. By combining an extended warranty with your instrument purchase, you'll extend the life of your investment and receive the support you need to keep your instrument running at peak performance.
Programs | Auto re-start (after Power Outages), Program Overwrite Protection |
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Thermal Uniformity | <0.5 °C (20 s after reaching 95°C) |
Block Configurations | 384-well, 0.02 mL |
Tm Calculator | Menu Driven through Touchscreen |
Reaction Volume Range | 5 to 20 μL |
Memory | USB and On-board |
User Interface | USB |
Max. Ramp Rate | 3.7°C/s (Block), 3.1°C/s (Sample) |
For Use With (Application) | PCR |
High-throughput Compatibility | High-throughput Compatible |
Thermal Range | 0°C to 100°C |
Sample Ramp Rate | ± 3.1°C⁄s |
Format | 384-well Plate |
Display Type | 6.5 in. VGA 32K Color Touchscreen |
Remarks | High-throughput, Single Block System optimized for Small Volume Reactions |
Capacity | 384 x 0.02 mL |
Reaction Speed | Standard |
Voltage | 100 to 240 V |
Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Temperature Range (Metric) | 4.0 to 99.9°C |
Peak Block Ramp Rate | 3.7°C⁄s |
Product Line | Veriti™ |
Applied Biosystems™ Veriti™ 96-Well Thermal Cycler
The Applied Biosystems™ Veriti™ 96-Well Thermal Cycler delivers the proven reliability of Applied Biosystems™ instruments.