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We offer a range of ultra pure, high-quality reagents for a variety of molecular biology applications such as nucleic acid electrophoresis, nucleic acid purification and sequencing, PCR and more.
From immunodetection reagents to protein purification, we offer a range of high-quality reagents that are pre-qualified for your protein research needs.
Browse Protein Chemistry Products
We offer a range of high-purity reagents that have been optimised for various cell and tissue culture applications including antibiotics, amino acids and media.
Fisher Bioreagents offers a wide range of reagents for Electrophoresis applications. Choosing the right reagents will allow you to achieve optimal results in your experiment. Use our quick selection guides to assist you in finding the right grades of Agarose, Buffers and Ladders.
Compared to polyacrylamide gel, agarose has a lower resolution but wider range of separation. Lower grades of agarose can be contaminated with other polysaccharides, salts and proteins. Such impurities can alter the gelling/melting temperature of agarose solutions or affect the ability to use the recovered nucleic acid sample in a post-electrophoresis application.
Browse all Fisher Bioreagents Agarose
Fisher BioReagents offers three different grades of agarose that are functionally tested and pre-qualified for specific applications. Agarose grades used in electrophoresis of nucleic acids:
Type of Agarose | Low EEO | Low Melting | Wide Separation Range | PCR Grade |
---|---|---|---|---|
Recovery of DNA and RNA | x | x | x | x |
Southern and Northern Biots | x | |||
DNA/RNA Separation 50bp to 1kb | x | |||
DNA/RNA Separation > 1kb | x | x | x | |
PCR Fragment Analysis | x | x | x | x |
In-gel Reactions (ligation, transformations, PCR) | ||||
Colony Lifts | x | |||
Available Pack Sizes | 100g to 500g | 25g | 100g to 500g | 100g |
Agarose Grade | Molecular Biology | Molecular Biology | Genetic Analysis | PCR |
Two buffers commonly used for DNA agarose electrophoresis are Tris-acetate with EDTA (TAE; 40mM Tris-acetate, 1mM EDTA) and Tris-borate with EDTA (TBE, 89mM Tris-borate, 2mM EDTA). TAE and TBE have different properties, which makes one more suitable than the other for a specific purpose.
Fisher BioReagents provides a wide range of standards, including routine DNA ladders for quick size and quality assessment as well as Fisher Bioreagentsâ„¢ exACTGeneâ„¢ DNA ladders that allow for quantitative analysis.
Browse all Fisher Bioreagents Ladders
Fisher Bioreagentsâ„¢ RiboLaddersâ„¢ RNA Standards
These standards can be used to assess single-stranded RNA molecules on both native and denaturing agarose gels. These unique RNA standards are lyophilized to reduce thawing-related degradation, to prolong shelf life and to ensure consistent performance.
exACTGene and Routine DNA Ladders
Ready-to-use (pre-mixed with the loading dye), room temperature, stable DNA ladders are available for all common electrophoresis applications.
Application | Size Range | Numbers of Bands | Number of Loadings |
---|---|---|---|
exACTGene DNA ladders are ideal for qualitative analysis, quantitative estimation, and size assessment | |||
PCR fragment analysis | 25-650bp | 14 | 100-10uL |
PCR fragment analysis, small DNA digests | 25-1,000bp | 12 | 100/10uL |
Quick check of PCR or enzyme digestion results | 50-2,000bp | 8 | 100/10uL |
General purpose, small DNA fragments | 100-1,000bp | 10 | 100/10uL |
Fast run times, small DNA fragments | 100-2,000bp | 8 | 100/10uL |
Clone identification | 100-2,686bp | 14 | 100/10uL |
Large size PCR or cloning | 100-2,000bp | 10 | 100/10uL |
Small or large cloning application | 100-5,000bp | 16 | 100/10uL |
General purpose, large digested DNA | 300-10,000bp | 13 | 100/10uL |
General purpose, wide size range | 100/10,000bp | 19 | 100/10uL |
General purpose, extra-large fragments | 300-24,000bp | 15 | 100/10uL |
Routine DNA ladders are designed for qualitative analysis and size assessment | |||
Small fragments, quick size assessment | 50-2,000bp | 11 | 200/5uL |
Quick size assessment of broad size range | 50-10,000bp | 16 | 200/5uL |