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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide
Brand: Invitrogen™ PV3180
This item is not returnable.
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Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains a PKC consensus sequence (R⁄K)1-3(X)0-2 (S⁄T) (X)0-2(R⁄K)1-3, where X is any residue.
Specifications
| Z´-LYTE™ | |
| FRET | |
| Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| Methylcoumarin | |
| 800 x 20 μL Assays | |
| PKC consensus sequence (R⁄K)1-3(X)0-2 S⁄T (X)0-2(R⁄K)1-3 | |
| 800 Reactions | |
| Dry Ice | |
| MAP4K5, IRAK4, PRKCQ, NEK9, PRKCD, MAP3K9, PASK, PIM2, NEK2, PRKCG, SRPK2, STK4, NEK1, MAP4K2, MINK1, CHEK2, PIM1, PKN1, PRKCA, PRKCI, TAOK2, MAP4K4, RPS6KB1, PHKG1, PRKCZ, ROCK1, PHKG2, PRKCB1, STK24, STK3, PRKCB2, STK25, PRKCE, PRKCH, MST4 |
| Serine/Threonine Kinases | |
| Serine/Threonine Kinase Assay | |
| Biochemical phospho-specific activity (no antibody) | |
| Kinase Assay Kit | |
| 384-well plate | |
| 405⁄465, 535 | |
| End Point | |
| Peptide-Based Substrate | |
| ROCK1, RPS6KB1, SRPK2, STK3, STK4, STK24, STK25, TAOK2, NEK2, NEK9, PASK, PHKG1, PHKG2, PIM1, PIM2, PKN1, PKC, CHEK2, IRAK4, MAP3K9, MAP4K2, MAP4K4, MAP4K5, MINK1, MST4, NEK1 |
For Research Use Only. Not for use in diagnostic procedures.