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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 17 Peptide
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.
Brand: Thermo Scientific™ PV4316
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Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7.
Specifications
| Z'-LYTE™ Ser/Thr 17 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Serine/Threonine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Serine/Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis | |
| Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acute-lyte™ kinase assays, enzyme and protein activity assays, protein assays and, FRET | |
| 800 x 20μL | |
| Biochemical phospho-specific activity (no antibody) | |
| Z'-LYTE™ Ser/Thr 17 Peptide Kinase Assay Kit | |
| 384-well plate | |
| Methylcoumarin | |
| End Point | |
| Dry Ice | |
| Peptide-Based Substrate | |
| Serine/Threonine Kinases |
| Z ́-LYTE™ | |
| Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer | |
| Serine/Threonine Kinase Assay | |
| –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| 800 x 20μL, 800 x 20 μL | |
| 405/465, 535 nm | |
| 800 Reactions | |
| Biochem J. vol 296, pp. 843-9 (1993) | |
| Similar to Ser-7 of Glycogen Synthase KKLNRTLSVA (KKLNRTLSFAEPG) | |
| CAMK2B, PRKCN, PRKD2, DCAMKL2, PRKD1, NEK4, MELK | |
| CAMK2B, DCAMKL2 , MELK, NEK4, PRKCN , PRKD1, PRKD2 |
For Research Use Only. Not for use in diagnostic procedures.