Vγ1.1 TCR Hamster anti-Mouse, Brilliant Violet 421, Clone: 2.11, BD

Description

The 2.11 monoclonal antibody recognizes the Vγ1.1 chain of the heterodimericγδT-cell receptor (TCRγδ) expressed by T lymphocytes and NK-T cells. It does not react with TCRαβ-bearing T cells. TCRγδassociates with CD3 molecules to form the TCRγδ/CD3 complex that mediates antigen recognition and can transduce intracellular signals leading to T cell responses. The Vγ1.1 TCR is also known as Vγ1 TCR. The Vγ1.1 variable region TCR gene segment primarily rearranges to join the Jγ4 and Cγ4 TCR gene segments. TCR Vγ1-expressing cells represent a major population of TCRγδcells in the adult mouse thymus, peripheral lymphoid tissues, and different epithelia. They constitute a minor population of TCRγδ-positive T cells during fetal and early postnatal life.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.