Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0

Description

Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Highlights:

• Three optimized binding buffers – for Protein A, Protein G or Protein A/G

• Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)

• Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose

Item-Specific Features:

• Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

• Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

• Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

• IgG Elution Buffer: pH 2.8; amine-based

• Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

• Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

• Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide

Includes:

Kits contain both binding and elution buffers