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Invitrogen™ Tango™ OPRK1-bla U2OS Cells
Tango™ OPRK1-bla U2OS Cells
Brand: Invitrogen™ K1576
This item is not returnable.
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Description
The Tango™ OPRK1-bla U2OS cells contain the human Opiod Receptor Kappa 1 (OPRK1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.The Tango™ OPRK1-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ OPRK1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
Specifications
| NM_000912 | |
| Dividing Cells | |
| Tail from AVPR2 GPCR | |
| OPRK1 | |
| Tango™ | |
| BLA (Beta-Lactamase) | |
| U2OS Cell | |
| Tango™ |
| Cell-based beta-arrestin recruitment | |
| K1576 Tango™ OPRK1-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ OPRK1-bla U2OS cells • Tango™ OPRK1-bla U2OS Assay Protocol • Certificate of Analysis Storage: The Tango™ OPRK1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescence | |
| Pool of Stable Cells | |
| 2 x 106 cells | |
| OPRK1 | |
| U2OS | |
| GPCR |
For Research Use Only. Not for use in diagnostic procedures.