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Thermo Scientific™ Tango™ GPR8-bla U2OS Cells
The Tango™ GPR8-bla U2OS cells contain the human Neuropeptides B/W receptor 2 (GPR8) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1478
This item is not returnable.
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Description
The Tango™ GPR8-bla U2OS cells contain the human Neuropeptides B/W receptor 2 (GPR8) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ GPR8-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of Neuropeptide B-29 (NPB29). In addition, Tango™ GPR8-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section.
Specifications
| NM_005286 | |
| Dividing Cells | |
| Tail from AVPR2 GPCR | |
| GPCRs | |
| GPR8-bla U2OS cells, GPR8-bla U2OS Assay Protocol, Certificate of Analysis | |
| Tango™ | |
| BLA (Beta-Lactamase) | |
| GPR8 | |
| U2OS | |
| GPCR |
| Cell-based beta-arrestin recruitment | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ GPR8-bla U2OS cells • Tango™ GPR8-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ GPR8-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescence | |
| GPR8 | |
| Pool of Stable Cells | |
| 2 x 106 Cells | |
| Room Temperature | |
| Dividing Cells | |
| Tango™ |
For Research Use Only. Not for use in diagnostic procedures.