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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.
Marke: IBA Lifesciences 5-4764-001
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Beschreibung
pYSG-IBA yeast expression vectorsThe pYSG-IBA vector allows the high-level expression of proteins in yeast.
- The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
- The expressed recombinant protein will be localized in the cytoplasm.
- High-level expression in yeast with high copy number
- Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
- Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
- URA3 auxotrophy marker for selection after transformation
Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Spezifikation
| Liquid | |
| pYSG-IBA 164 | |
| leu2-d and URA3 | |
| CUP1 | |
| 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg | |
| Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal | |
| 5 μg | |
| Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins |
| StarGate™ System, Yeast Host Cells | |
| Copper sulfate | |
| 8 | |
| Acceptor Vector for yeast expression | |
| 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| 250 ng/μL | |
| Clear |