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  5. IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

Product Code. 15653838 Shop All IBA Lifesciences Products
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Vector:
pLSG-IBA 102
pLSG-IBA 103
pLSG-IBA 104
pLSG-IBA 105
pLSG-IBA 123
pLSG-IBA 142
pLSG-IBA 143
pLSG-IBA 144
pLSG-IBA 145
pLSG-IBA 162
pLSG-IBA 164
pLSG-IBA 167
pLSG-IBA 168
pLSG-IBA 23
pLSG-IBA 25
pLSG-IBA 3
pLSG-IBA 33
pLSG-IBA 35
pLSG-IBA 43
pLSG-IBA 45
pLSG-IBA 5
pLSG-IBA 62
pLSG-IBA 63
pLSG-IBA 64
pLSG-IBA 65
pLSG-IBA wt1
pLSG-IBA wt2
Unit Size:
5µg
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Product Code. 15653838

Brand: IBA Lifesciences 54865001

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Beskrivning

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.

pLSG-IBA Baculo virus (insect cells) expression vectors

The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.

  • Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
  • Cloning and expression of recombinant proteins in insect cells
  • Polyhedrin promoter drives high-level expression of the insert
  • Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli

Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifikationer

Format Liquid
For Use With (Equipment) StarGate™ System, Insect Host Cells
Inducing Agent BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies)
Includes pUC ori, Ampicillin resistance
pH 8
Promoter Polyhedrin
Product Type Acceptor Vector for bacula (insect cell) expression
Storage Buffer 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Content And Storage 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein Tag FLAG-tag for N-terminal
Concentration 250 ng/μL
Quantity 5 μg
Vector pLSG-IBA 65
Color Clear
For Use With (Application) Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
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