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Gibco™ RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand: Gibco™ 42402016

Product Code. 11574496

27.79€ / 500mL

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Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

With: Phenol Red, HEPES
Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

Certifications/Compliance	ISO 13485 standards, FDA
Culture Environment	CO₂
Classification	Animal Origin-free
Culture Type	Mammalian Cell Culture
Product Type	RPMI 1640
Sterility	Sterile-filtered
Without Additives	No Glutamine, No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
pH	7 to 7.4
Quantity	500 mL

Content And Storage	Storage: 2°C to 8° C, protect from light Shipping: Ambient Shelf life: 12 months from date of manufacture
Description	Roswell Park Memorial Institute (RPMI) 1640 Medium, 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Phenol Red, HEPES, 12 Months Shelf Life, 270 to 300mOsm/kg Osmolality, For Mammalian Cell Culture
Concentration	1X
Form	Liquid
Serum Level	Standard Serum Supplementation
With Additives	HEPES, Phenol Red, Sodium Bicarbonate
For Use With (Application)	Mammalian cell culture
Osmolality	270–300 mOsm/kg
Product Line	Gibco™
Shipping Condition	Room Temperature

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Gibco™ RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine

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