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  8. 11554496

Gibco™ RPMI 1640 Medium, HEPES, no glutamine
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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ 42401018

21.26 EUR valid until 2024-12-31
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Product Code. 11554496

  • 26.60€ / 500mL

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Description

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

With: Phenol Red, HEPES

Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Mammalian Cells
1 X
Liquid
Standard Serum Supplementation
HEPES, Phenol Red, Sodium Bicarbonate
cGMP-compliant under the ISO 13485 standard
7 to 7.4
500 mL
CO2
Animal Origin-free
Mammalian Cell Culture
RPMI 1640
Sterile-filtered
No Glutamine, No Sodium Pyruvate
270 - 310 mOsm/kg
Gibco™
Room Temperature
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.