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Invitrogen™ RiboMinus™ Pan-Prokaryote Probe Mix
The RiboMinus™ Pan-Prokaryote Probe Mix is a pool of more than 150 3'-biotin labeled ssDNA oligonucleotides that are used to capture and eliminate bacterial and archaebacterial ribosomal RNA from RNA samples in preparation for NGS transcriptome studies.
Brand: Invitrogen™ A46920
This item is not returnable.
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Description
RiboMinus™ Pan-Prokaryote Probe Mix is a component of the RiboMinus Bacteria 2.0 Transcriptome Isolation Kit and is available as a standalone product for use in conjunction with other RiboMinus products targeting other species: Plant (Cat. No. A1083808) and Eukaryote v2 (Cat. No. A15026). Combining RiboMinus Pan-Prokaryote Probe Mix with Plant or Eukaryote probe mixes is useful for metatranscriptomic studies. This probe mix consists of over 150 3’-biotin-labeled ssDNA oligonucleotide designs. These designs were created using highly conserved regions of rRNA across 76 pan-prokaryote species (see table below) and tested with several species (see compatibility list below).
Specifications
| RiboMinus Isolation kits include RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored. | |
| 1.25 hr. | |
| Transcriptome RNA | |
| Next Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction | |
| RiboMinus™ | |
| 100 ng to 5 ug of total RNA | |
| RiboMinus |
| Magnetic Bead | |
| Total RNA (Bacterial) | |
| Not High-throughput Compatible (Manual) | |
| 12 | |
| 1 | |
| Transcriptome RNA | |
| Magnetic Bead |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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