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R&D Systems™ Recombinant Human His6-USP1/His6-UAF1 Complex Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ E-568-050
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Description
Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.Specifications
| O94782.1 (USP1), Q8TAF3.1 (UAF1) | |
| Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol. | |
| M.W. (theoretical): 89 kDa (USP1), 77 kDa (UAF1) | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening. | |
| Unconjugated | |
| >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain |
| Bioactivity | |
| 7398 | |
| His6 USP1/UAF1 Complex | |
| Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein Met1 - Leu785 (Gly670Ala, Gly671Ala) with a C-terminal 6-His tag (USP1), Met1 - Thr677 with a C-terminal 6-His tag (UAF1) | |
| USP1 | |
| Recombinant | |
| USP1/UAF1 Complex |
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