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Proteintech™ Rat IL-1 beta ELISA Kit
KE20005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 beta.
Brand: Proteintech™ KE20005-96T
This item is not returnable.
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Description
- The assay recognizes rat IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells.
- The IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 beta is added to detect the captured IL-1 beta protein.
- For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL1 gene family encodes three proteins: IL1 -alpha IL1- beta and their naturally occurring inhibitor IL1-RN. Interleukin 1 beta(IL1- beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation.
- IL1-beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL1-beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Specifications
| NP_113700 | |
| 12.5 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Rat | |
| Rat | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 31.25 to 2000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 24494 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| Q63264 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Rat IL-1 beta ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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