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Thermo Scientific™ Pierce™ Rapid Antibody Isotyping Kit - Mouse
Tienda Thermo Scientific ProductosDescripción
Thermo Scientific™ Pierce Rapid Mouse Antibody Isotyping Kits are single-use, cassette-based kits for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.
The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.
Highlights:
Long shelf life – stable for more than 1 year at room temperature
Single-step – add diluted antibody sample to the loading-well of cassette
Fast – within 5 minutes a band appears that indicates the antibody isotype
Sensitive – detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL
Specific – assay does not crossreact with fetal bovine serum (FBS)
Reliable – results are similar to the standard ELISA-based isotyping assays
Includes:
Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent
Especificaciones
Especificaciones
| Description | Pierce Rapid Isotyping Kit - Mouse |
| Content And Storage | Sufficient For: 10 isotype determinations • Isotyping Cassettes (two per pouch), 10 pouches • Sample Diluent, 45 mL Store kit at 2–8°C. |
| For Use With (Application) | Membrane Blot |
| Quantity | 10 Tests |
| Assay | Isotyping Assay |
| Detection Method | Chromogenic |
| Product Line | Pierce |
| Target Specificity | Target-specific |
For Research Use Only. Not for use in diagnostic procedures.
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