Ready-to-use solution of o-phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent to measure peptides or amino acids (Ex 340nm, Em 455nm).
Brand: Thermo Scientific™ 26025
Thermo Scientific™ Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.
Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.
Highlights:
For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives
Provides an accurate measure of both composition and absolute protein-peptide content
Ideal for work with recombinant proteins and synthetic peptides
Can be used for fluorescent protein or peptide assay
Pre-column derivatization mixtures can be injected into LC without any processing
Reagent Properties:
Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10
Form: Clear, colorless to slightly yellow liquid
Reactive toward: Primary amines (–NH2)
Detection: Fluorescence (excitation/emission = 340nm/455nm)
Application: Pre- or post-column derivatization for detection by HPLC
| o-Phthaldialdehyde | |
| Fluoraldehyde™ | |
| OPA (o-phthaldialdehyde) | |
| 945 mL |
| Amine | |
| Store under nitrogen at 4°C away from oxidizing reagents and direct sunlight. | |
| Fluorescence | |
| Liquid |
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For Research Use Only. Not for use in diagnostic procedures.
