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Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit
Chemically conjugate any two purified proteins (antibodies, enzymes, carriers, etc.) using sulfo-SMCC crosslinker, a complete kit with modification reagents and buffers.
Brand: Thermo Scientific™ 23456
This item is not returnable.
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Description
Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.
The kit provides Sulfo-SMCC crosslinker for amine-to-sulfhydryl conjugation and SATA modification reagent for specific sulfhydryl-addition. The crosslinking strategy allows two purified proteins, such as an antibody and a reporter enzyme, to be conjugated in a two-step, controlled manner. Supplementary components, buffers and columns are also included to support successful creation of a useful conjugate via any one of several specific protocols.Highlights:
Sulfo-SMCC – high-quality crosslinker conjugates primary amines of protein 1 to sulfhydryl groups of protein 2
SATA or 2-MEA – modification reagents provide two options for making sulfhydryl groups available on protein 2 (usually an antibody)
Ellman's Reagent – provides a mean of monitoring and assessing efficiency of the final reaction
Complete – includes critical reagents as well as buffers, desalting columns and supplementary modification reagents
Specifications
| Amine-Sulfhydryl | |
| Controlled Protein-Protein Crosslinking Kit | |
| No | |
| Sufficient For: One conjugation with two purified proteins (up to 5 mg each) • Sulfo-SMCC Crosslinking Agent, 2 mg • Activation/Conjugation Buffer (10X), 20 mL • PBS packs (each makes 500mL), 2 packs • 2-Mercaptoethylamine-HCl, 6 mg • Immobilized-Reductant, 0.2 mL • SATA, 2 mg • Dimethylformamide (DMF), 1 mL • Hydroxylamine-HCl, 5 mg • Dextran Desalting Column, 2 x 10 mL • Column Extender, 2 ea. • Cysteine-HCl, 20 mg • Ellman's Reagent, 2 mg Store at 4°C. |
|
| Ambient | |
| Chemical Labeling | |
| Maleimide, Sulfo-NHS Ester | |
| Kit | |
| Water | |
| Yes |
| No | |
| 436.37 | |
| 11.6 Å | |
| No | |
| Pierce™ | |
| Heterobifunctional | |
| Short (<10 Å) | |
| 1 Reaction | |
| Kit | |
| Crosslinker |
For Research Use Only. Not for use in diagnostic procedures.