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Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media
Used for capture and intermediate purification requiring low to medium hydrophobicity
Brand: Cytiva 17-0973-10
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Description
- Base matrix derivatized via uncharged, chemically-stable ether linkages
- Two levels of substitution help find optimal selectivity and binding capacity for given application
- Hydrophilic nature of the base matrix ensures low levels of non-specific binding
- Hydrophobic interaction chromatography (HIC) media composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages
- Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
- Degree of substitution for low sub HIC media is ∽25μmol phenyl/mL gel
- Degree of substitution for high sub HIC media is ∽40μmol phenyl/mL gel
Specifications
| 25 mL | |
| For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity | |
| Phenyl Sepharose 6 Fast Flow Media (high sub) | |
| 45 to 165 μm | |
| 3 to 13 | |
| Phenyl |
| Stable in commonly used aqueous Buffers: 1 M NaOH, 3 M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6 M Guanidine-Hydrochloride, 8 M Urea | |
| 4°C to 30°C | |
| 250 to 400 cm/Hoμr | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Phenyl Sepharose 6 Fast Flow (high sub)
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P240-Ground/bond container and receiving equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol