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Novus Biologicals™ PC-3 Whole Cell Lysate
for use as a positive control in Western Blot
Brand: Novus Biologicals™ NBP3-06995
This item is not returnable.
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Specifications
| This product has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 uL depending on the size format of your gel. | |
| Multi-step | |
| PC-3 |
| 500 μg | |
| Store at -70C. Avoid freeze-thaw cycles. |