Thermo Scientific™ IMAGEN™ Parainfluenza Virus Group Kit using Direct Immunofluorescence Assay

Description

Rapidly detect Parainfluenza virus types 1, 2 and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations with Thermo Scientific™ IMAGEN™ Parainfluenza Group Kit. This direct immunofluorescence assay utilizes type-specific monoclonal antibodies to detect and identify Parainfluenza virus types 1, 2 and 3.

Parainfluenza viruses are members of the genus Paramyxovirus classified within the family Paramyxoviridae.¹ There are 6 species of Paramyxovirus known to infect hummans which include Parainfluenza viruses 1, 2, 3, and 4 (subtypes 4a and 4b), Mumps virus, and Newcastle Disease virus. Of the 4 Parainfluenza viruses, types 1, 2, and 3 are now recognized as a major cause of acute respiratory disease in infants and children.^2,3

Use IMAGEN Parainfluenza Kit as a rapid, sensitive, and specific method for detection of Parainfluenza virus types 1, 2, and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations.

• Rapid results—within a little over 30 minutes of receiving test sample or culture
• Simple—ready-to-use reagents plus all IMAGEN assays follow the same basic methodology
• Excellent sensitivity and specificity—high quality, well-characterized antibodies used in the assay provide excellent sensitivity and specificity
• Easy-to-read results
• Long shelf life—24 months

The IMAGEN Parainfluenza Kit contains fluorescein isothiocyanate (FITC) conjugated monoclonal antibodies which bind specifically to either Parainfluenza virus types 1, 2, or 3. Specimens are incubated with the FITC conjugated reagents for 15 minutes. Excess reagent is then removed by washing with phosphate-buffered saline (PBS). The stained areas are mounted and viewed using epifluorescence illumination. If Parainfluenza virus types 1, 2, or 3 are present then characteristic apple-green intracellular fluorescence is seen within infected cells, which contrasts with the red background staining of uninfected cells.

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