Invitrogen™ NuPAGE™ Midi Bis-Tris Welcome Pack for Protein Expression (4–12%, 20 well)

Description

The NuPAGE Midi Bis-Tris Welcome Pack provides all necessary NuPAGE Bis-Tris gels, buffers, and reagents needed to begin using the SureLock Tandem Midi Gel Tank for your protein separation and visualization needs.

Key features include:

• Rapid time to results—NuPAGE Bis-Tris Midi gels afford a 35-minute run time and can be stored at room temperature for up to 12 months
• Neutral-pH run environment that minimizes protein modifications
• Quick 12-minute staining protocol using the SimplyBlue SafeStain for target protein visualization

The NuPAGE Midi Bis-Tris Welcome Pack for Protein Expression contains:

• SureLock Tandem Midi Gel Tank
• NuPAGE 4-12% Bis-Tris Midi Gels, 20-wells (2 boxes, 20 gels)
• NuPAGE MES SDS Running Buffer (20X)
• NuPAGE LDS Sample Buffer (4X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
• SimplyBlue SafeStain

About the SureLock Tandem Midi Gel Tank

The SureLock Tandem Midi Gel Tank enables rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a setup time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. With 26-well gels up to 52 samples can be run at once for high throughput applications. The tank is compatible with all Invitrogen pre-cast midi gels and midi gel cassettes.

About NuPAGE 4-12% Bis-Tris Midi Gels

NuPAGE Bis-Tris Midi protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine midi gels, NuPAGE Bis-Tris midi gels have a 12-month shelf life at room temperature with a neutral pH environment that minimizes protein modifications during the gel run. NuPAGE Bis-Tris Midi gels can be used for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE midi gels for optimal results during day-to-day use. The NuPAGE 4-12% Bis-Tris midi gel has a 4–12% gradient polyacrylamide matrix that will separate a broad range of protein molecular weights from 2.5 to 260 kDa using the supplied MES buffer.

About SimplyBlue SafeStain

SimplyBlue Safe Stain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels. The SimplyBlue SafeStain protocol is easy to perform and can be completed in just 12 minutes using a simple microwave procedure. Destaining is not required but may be performed with deionized water to achieve maximum sensitivity. This stain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains creating a healthier lab environment.