missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ Recombinant Protein G Agarose
For the purification of IgG from most mammalian species and does not bind IgM, IgA, IgE or serum albumin
Brand: Invitrogen™ 15920010
Includes: rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol.
Description
- Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol)
- The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads
- rProtein G Agarose has a binding capacity of approximately 20mg of human IgG permL of beads
- rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A
- The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography
- To purify IgG, load crude samples (serum, ascites fluid, or hybridoma supernatant) onto a column of rProtein G Agarose under physiologic conditions of salt and pH
- Non-IgG components do not bind to the beads and elute in the flow through fraction; Purified IgG is then eluted with a low pH buffer
PCR, PCR and Real-Time PCR
Order Info
Shipping Condition: Wet Ice
Specifications
| Affinity Column | |
| Agarose Gel | |
| Suspension | |
| Protein G |
| rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol. Store at +4°C. | |
| Protein | |
| Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.