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Thermo Scientific Chemicals MOPS-SDS running buffer (20X)
MOPS-SDS running buffer (20X) is a buffer frequently used for separating medium- to large-sized proteins on a gel.
Brand: Thermo Scientific Chemicals J62847.K2
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Description
MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. MOPS-SDS Running Buffer is suitable for protein gel electrophoresis. Use of MOPS buffer allows proteins to run slower than when using MES buffer. This buffer is used in 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis - See more at:
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- MOPS-SDS running buffer is a zwitterionic, morpholinic buffer that allows the separation of medium- to large-sized proteins
Application
- MOPS is used as a running buffer in electrophoresis. The use of MOPS buffer allows proteins to run slower than when MES buffer is used
- MOPS-SDS running buffer, 20X concentrated, is formulated to be used for running proteins on an SDS gel
- This buffer contains SDS detergent to maintain the denaturation of proteins and MOPS to buffer the pH
- It is commonly used for cell culture media and protein purification in chromatography
- MOPS is frequently applied in buffered culture media for bacteria, yeast, and mammalian cells
- It is also applied in the bicinchoninic acid (BCA) assay
- It is used in separating RNA in agarose gels
Specifications
| MOPS-SDS running buffer | |
| Liquid | |
| It is soluble in water. |
| 20X | |
| 1 L |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- MOPS-SDS running buffer (20X)
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : Sodium lauryl sulfate
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