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Invitrogen™ Click-IT™ Tetramethylrhodamine (TAMRA) Protein Analysis Detection Kit
Supports differential analysis of glycoprotein subclasses, total proteins and total glycoproteins in the same gel
Brand: Invitrogen™ C33370
Description
The Click-iT Tetramethylrhodamine (TAMRA) Glycoprotein Detection Kit provides the second part of the simple and robust two-step technique to identify and characterize glycoproteins by one- or two-dimensional gel electrophoresis. In step two, after the incorporation of the azide handle into protein glycan structures with either a Click-iT metabolic labeling reagent or the Click-iT Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. Gels with TAMRA labeled glycoproteins can be subsequently stained with the Multiplexed Proteomics™ technologies, SYPRO™ Ruby total protein stain and PRO-Q™ Emerald glycoprotein stain for the differential analysis of glycoprotein subclasses, total proteins and total glycoproteins in the same gel. Click-iT modified glycoproteins are also compatible with downstream LC-MS/MS and MALDI MS analysis for identification.
- Less hazardous
Cell Analysis, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
Less hazardous | |
TAMRA Protein Analysis Detection Kit | |
Fluorescence | |
TAMRA™ Isomers, TMR (Tetramethylrhodamine) | |
Proteins (Glycoproteins) |
1 kit | |
Store in freezer -5°C to -30°C. | |
Room Temperature | |
Click-iT™ |
For Research Use Only. Not for use in diagnostic procedures.