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Molecular Probes™ LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit, for flow cytometry
The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types.
Brand: Molecular Probes™ L34856
This item is not returnable.
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Description
The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of microspheres for accurate sample volume measurements.
Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.
Specifications
| Contains 1 vial SYTO™ 9 nucleic acid stain (200 μL, 3.34 mM in DMSO), 1 vial of propidium iodide (200 μL, 20 mM in DMSO), and 1 vial of a microsphere standard (1.0 mL of 6.0 μm diameter microspheres). Store in refrigerator (2–8°C) and protect from light. |
|
| Tube | |
| SYTO™ 9, propidium iodide | |
| 100 reactions | |
| SYTO™ 9: 485/498, PI: 535/617 |
| Fluorescence | |
| Bacterial Viability and Counting Kit | |
| BacLight™, LIVE/DEAD™ | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.