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Thermo Scientific™ LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat
The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment.
Brand: Thermo Scientific™ PV4688
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Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15137:
The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.
When running the LanthaScreen™ TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Specifications
| Tb (Terbium), FITC (Fluorescein) | |
| Fluorescence | |
| RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers | |
| For research use only (RUO) | |
| The LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit contains RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C). |
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| End Point | |
| RAR beta, NR1B2, RARB | |
| Co-Factor Interaction Assay, TR-FRET | |
| TR-FRET RAR Beta Coactivator Assay Kit |
| LanthaScreen™ TR-FRET RAR Beta Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, FITC (Fluorescein), Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification | |
| Microplate Reader | |
| 800 x 20 μL Assays | |
| 384-well Plate | |
| Biochemical coregulator interaction | |
| Dry Ice | |
| LanthaScreen™ | |
| PAR beta | |
| 800 x 20 μL Assays |
For Research Use Only. Not for use in diagnostic procedures.