missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. DNA Polymerases
  6. Invitrogen™ T4 DNA Polymerase

Invitrogen™ T4 DNA Polymerase

DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity

258.00€ - 734.00€

Specifications

Content And Storage T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.
Description DNA Polymerase
Polymerase T4 DNA Polymerase
Exonuclease Activity 3' - 5'
Shipping Condition Wet Ice
View More Specs

Includes: T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165mM Tris acetate (pH 7.9), 330mM sodium acetate, 50mM magnesium acetate, 5mM DTT].

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10164552
View Documents
missing translation for 'viewProductCertificates'
Invitrogen™
18005017 		50 U
258.00€
Each
Please to purchase this item. Need a web account? Register with us today!
10043202
View Documents
missing translation for 'viewProductCertificates'
Invitrogen™
18005025 		250 U
734.00€
Each
Please to purchase this item. Need a web account? Register with us today!
Description

Description

  • Applications: Labeling double-stranded linear DNA by replacement synthesis, Oligonucleotide-directed, site-specific mutagenesis, 3' end-labeling of double-stranded DNA, Polishing both 5' or 3 overhangs to make blunt ends
  • Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid
  • Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested
  • Unit Definition: One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template primer
  • Unit Reaction Conditions: 50mM glycine-NaOH (pH 8.8), 16.6mM (NH4)2SO4, 6mM MgCl2, 6.5μM EDTA, 10mM 2-mercaptoethanol, 0.165mg/mL BSA, 1.6mg/mL DNase I-nicked salmon testes DNA, 0.33mM dCTP, 0.33mM dATP, 0.33mM dGTP, 0.33mM dTTP, 76nM [3H]dTTP, and enzyme in 0.1mL for 30 min. at 37°C
  • A T4 DNA Polymerase Technical Bulletin is available

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

Specifications

Specifications

T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.
T4 DNA Polymerase
Wet Ice
DNA Polymerase
3' - 5'
No
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.