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Invitrogen™ T4 DNA Ligase (1 U/μL)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Brand:  Invitrogen™ 15224017

47.00 EUR valid until 2025-03-21
Use promo code "24111" to get your promotional price.

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Product Code. 10124752

  • 70.50€ / Each

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Includes: Vial of 5X reaction buffer (250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000)
This item is not returnable. View return policy

Description

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.

  • Optimizes ligation in 5 minutes
  • Source: Purified from E. coli lambda lysogen NM989
  • Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
  • Unit definition: One unit catalyzes exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
  • Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2, 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning

Order Info

Shipping Conditions: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

1 U/μL
Dry Ice
DNA Ligase Buffer, 5X Reaction Buffer
T4 DNA Ligase
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
Ligase
100 U
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T4 DNA Ligase 1U/ul Version: 052002 File size: 101.3 kB
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2986797 Certificate TypeCertificate of Analysis Date30/01/2025 Catalog Number15224017
Lot Number2478344 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number15224017
Lot Number2660116 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number15224017
Lot Number2839509 Certificate TypeCertificate of Analysis Date27/01/2025 Catalog Number15224017
Lot Number2945669 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog Number15224017
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