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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit
The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.
Brand: Invitrogen™ K210006
207.10 EUR valid until 2024-12-31
Use promo code "21745" to get your promotional price.
This item is not returnable.
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Description
The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.
- Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
- High-purity, low-endotoxin product- pure enough for mammalian cell transfection
- Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
- PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
- Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal
- Plasmid preparations can typically be completed in less than 2 hours
Contaminant-free DNA
- Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
- Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
- Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
- Purification time is two hours
Order Info
Shipping Condition: Room Temperature
Specifications
| • 100 mL Resuspension Buffer (R3) • 550 μL RNase A • 100 mL Lysis Buffer (L7) • 100 mL Precipitation Buffer (N3) • 300 mL Equilibration Buffer (EQ1) • 2 × 300 mL Wash Buffer (W8) • 200 mL Elution Buffer (E4) • 30 mL TE Buffer • 10 HiPure Columns • 3 Column Holders Store all components at room temperature. |
|
| Anion Exchange Resin | |
| Maxi | |
| BAC DNA, Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| <40kb, Low Copy Plasmid, High Copy Plasmid, BAC | |
| PureLink™ | |
| 10 Preps | |
| Room Temperature | |
| PureLink™ | |
| 1000 μg |
| Column | |
| Bacterial Culture | |
| 15 mL | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| 10 Preps | |
| > 200 μg (Large-Scale) Plasmid DNA | |
| Plasmid MaxiPrep Kit | |
| Transfection Grade | |
| BAC DNA, Plasmid DNA | |
| 2 hr. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- PureLink HiPure Plasmid Maxiprep Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
Supplemental information
- MIXTURE LIST-Contains : acetic acid
For Research Use Only. Not for use in diagnostic procedures.