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Invitrogen™ ProQuest™ Two-Hybrid System with Gateway™ Technology
An in vivo yeast-based system for identifying interactions between two proteins
Brand: Invitrogen™ PQ1000101
1104.32 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
Includes: The ProQuest Two-Hybrid System includes yeast expression vectors pDEST32, pDEST22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay.
Description
- Reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media
- Allow rapid and easy generation of bait and prey constructs, and facilitate downstream application
- Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
- Three different reporter genes (HIS3, URA3, and lacZ) with independent promoter regions to rapidly weed out false positives
- URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid
- A set of strong-to-weak Gateway-based control interactions to evaluate results and assess the strength of the interaction being tested
Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis, Yeast Two-Hybrid
Specifications
| Two-Hybrid System | |
| The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored. | |
| Yeast | |
| Gateway™, ProQuest™ |
| Beta-Gal (lacZ), HIS3, URA3 | |
| Two-Hybrid Assay | |
| 1 kit |
For Research Use Only. Not for use in diagnostic procedures.