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Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Brand: Invitrogen™ 10928042
658.69 EUR valid until 2024-09-28
Use promo code "21626" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21626” to get your promotional price
Description
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.
All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.
Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat. No. 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat. No. 12595025). The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.
Specifications
• SuperScript II RT/Platinum Taq DNA Polymerase mix (100 μL) |
|
| Kit | |
| Platinum Taq DNA Polymerase | |
| 1-step RT-PCR | |
| SuperScript II | |
| Wet Ice | |
| Built-In Hot Start | |
| Master Mix | |
| Up to 7 kb |
| Gel Electrophoresis | |
| 1-step RT-PCR | |
| 30 to 50 min. | |
| 55°C to 60°C | |
| Reduced | |
| PCR Amplified cDNA | |
| 100 Reactions | |
| Reverse Transcription | |
| RNA |
For Research Use Only. Not for use in diagnostic procedures.