missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ pBAD/His Kit
Express the protein of interest with an N-terminal tag in a tightly regulated fashion
Brand: Invitrogen™ V43001
1013.20 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: 20μg of each pBAD/His A, B & C vector, 20μg of pBAD/His/lacZ, 1mL of 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- araBAD promoter for tightly regulated expression
- Translation initiation signals optimized for E. coli expression
- N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody
- N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody
- Enterokinase cleavage site for removing the N-terminal tag following purification
- Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Inducible | |
araBAD | |
None | |
Ampicillin (AmpR) | |
His Tag (6x) | |
1 kit |
Arabinose | |
Bacterial Expression Vector | |
The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. | |
EK (Enterokinase) Recognition Site | |
Restriction Enzyme/MCS | |
pBAD |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- pBAD/His Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362-Take off contaminated clothing.
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction