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Invitrogen™ pBAD/His Kit

Express the protein of interest with an N-terminal tag in a tightly regulated fashion

Brand:  Invitrogen™ V43001

1013.20 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.



Product Code. 10552443

  • 1192.00€ / 1 set

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Includes: 20μg of each pBAD/His A, B & C vector, 20μg of pBAD/His/lacZ, 1mL of 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
This item is not returnable. View return policy

Description

Description

  • araBAD promoter for tightly regulated expression
  • Translation initiation signals optimized for E. coli expression
  • N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody
  • N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody
  • Enterokinase cleavage site for removing the N-terminal tag following purification
  • Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Inducible
araBAD
None
Ampicillin (AmpR)
His Tag (6x)
1 kit
Arabinose
Bacterial Expression Vector
The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
EK (Enterokinase) Recognition Site
Restriction Enzyme/MCS
pBAD
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Videos
Safety and Handling

Safety and Handling

Product Identifier
  • pBAD/His Kit

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362-Take off contaminated clothing.

SDS
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For Research Use Only. Not for use in diagnostic procedures.