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Invitrogen™ pBAD/gIII Kit
Express recombinant proteins under the tight regulation of the araBAD promoter
Brand: Invitrogen™ V45001
856.44 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
- Features araBAD promoter for tightly regulated expression
- Translation initiation signals for optimized E. coliexpression
- Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
- C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
- A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| Restriction Enzyme/MCS | |
| pBAD |
| Arabinose | |
| Bacterial Expression Vector | |
| The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored. | |
| c-Myc Epitope Tag | |
| 1 kit |
For Research Use Only. Not for use in diagnostic procedures.