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Invitrogen™ Expressway™ Maxi Cell-Free E. coli Expression System
Uses efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4 to 6 hours
Brand: Invitrogen™ K990097
Includes: Designed to perform 200 x 25μL,5 x 1mL, or 1 x 5mL (initial volume) reactions or one to several large reactions. Includes the E.coli slyD- Extract, 2.5X IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, T7 Enzyme Mix, and a positive expression control vector. The Expressway Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO and pEXP5-CT/TOPO also includes the pEXP5-NT/TOPO and pEXP5-CT/TOPO TA Expression Kits. Each kit contains the pEXP5-NT/TOPO or pEXP5-CT/TOPO vector, other reagents to facilitate TOPO Cloning, and TOP10 Chemically Competent E. coli.
Description
- Procedure can be performed in single reaction tube and is easily scalable without need for specialized equipment
- TOPO TA Cloning expression vectors (5-min cloning with 95% efficiency) are provided for optimal expression results
- In addition to all advantages of open expression system, produces high levels of recombinant protein that may be easily detected and purified for various downstream applications
- Proprietary formulation of Feed Buffer boosts reaction productivity so that more than one milligram of protein is produced in 2mL reaction within 4 to 6 hours
- The formulation of lysate enables protein synthesis using either circular or linear templates
- One kit is supports 200 x 50 reactions, which can be accommodated by 2 x 96-well plates
- Without feed buffer, such reactions need only 2 hours
- Once positive expression is detected, scale-up protein production can be performed with large cell-free format or in E. coli cell-based systems
Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| T7 | |
| The Expressway™ Maxi Cell-Free Expression System is designed to perform two hundred 25-μl, five 1-ml, or one 5-ml (initial volume) reactions or one to several large reactions, depending on your needs. This system includes the E.coli slyD- Extract, 2.5X IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, T7 Enzyme Mix, and a positive expression control vector. The Expressway™ Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO™ and pEXP5-CT/TOPO™ also includes the pEXP5-NT/TOPO™ and pEXP5-CT/TOPO™ TA Expression Kits. Each kit contains the pEXP5-NT/TOPO™ or pEXP5-CT/TOPO™ vector, other reagents to facilitate TOPO™ Cloning, and One Shot™ TOP10 Chemically Competent E. coli. Store the vectors at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored. | |
| Cell-Free | |
| N-Terminal | |
| 1 kit | |
| Expressway™ |
| Cell-Free Expression System | |
| Water Soluble Protein | |
| Cell-Free Expression | |
| TOPO-TA | |
| pEXP5-NT/TOPO, pEXP5-CT/TOPO |
For Research Use Only. Not for use in diagnostic procedures.