missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. DNA Polymerases
  6. 10605013

Invitrogen™ DNA Polymerase I/DNase I

An optimized mixture of both enzymes for efficient nick translation of DNA

Brand:  Invitrogen™ 18162016

Product Code. 10605013

  • 304.00€ / Each

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

Includes: DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.
This item is not returnable. View return policy

Description

Description

  • Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
  • Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
  • Performance and Quality Testing: Performance tested in nick translation reaction
  • Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
  • Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template•primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min. at 37°C

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.
DNA Polymerase I
250 U
No
DNA Polymerase
3' - 5', 5' - 3'
Wet Ice
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Product Information

Manuals

DNA Polymerase I/ DNase 1 Version: MAN0001314 1.01 (27 Dec 01) File size: 49.1 kB
Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

2 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2835649 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number18162016
Lot Number1630619 Certificate TypeCertificate of Analysis Date19/07/2017 Catalog Number18162016
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Invitrogen™ DNA Polymerase I/DNase I > 250 units

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.