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Invitrogen™ DNA Polymerase I

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

261.00€ - 782.00€

Specifications

Description DNA Polymerase
Polymerase DNA Polymerase I
Exonuclease Activity 3' - 5', 5' - 3'
Shipping Condition Wet Ice
Hot Start No
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Includes: 1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5-10 units/μL.

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10358002
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Invitrogen™
18010017 		250 U
261.00€
Each
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10043132
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Invitrogen™
18010025 		1000 U
782.00€
Each
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Description

Description

Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions
50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.

  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
  • Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

Specifications

Specifications

DNA Polymerase
3' - 5', 5' - 3'
No
DNA Polymerase I
Wet Ice
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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