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Invitrogen™ AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
546.00€ - 2555.00€
Specifications
| Detection Method | Primer-probe |
|---|---|
| GC-Rich PCR Performance | Low |
| Polymerase | AccuPrime Pfx DNA Polymerase |
| Reaction Speed | Standard |
| Shipping Condition | Wet or Dry Ice |
Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
10472482
|
Invitrogen™
12344024 |
200 Reactions |
546.00€
Each |
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|
10227242
|
Invitrogen™
12344032 |
1000 Reactions |
2555.00€
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
- Greater than 26 times higher fidelity than Taq DNA polymerase
- Amplification of fragments up to 12 kb
- Minimized PCR optimization
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- High-fidelity PCR
- Hot-start PCR
Notes
- AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
- The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
| Primer-probe | |
| AccuPrime Pfx DNA Polymerase | |
| Wet or Dry Ice | |
| 26X | |
| Blunt | |
| 12 kb or less |
| Low | |
| Standard | |
| Hot-start PCR, High-fidelity PCR | |
| Built-In Hot Start | |
| Separate Components |
For Research Use Only. Not for use in diagnostic procedures.