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Thermo Scientific™ GeneJET Stabilized and Fresh Whole Blood RNA Kit
Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
Brand: Thermo Scientific™ K0871
226.67 EUR valid until 2025-03-29
Use promo code "24111" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Description
The GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).
Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).
Highlights
- Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
- Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
- Efficient—up to 4 μg of total RNA from 500 μL of healthy human blood
- Pure—isolates intact total RNA free of any RT-qPCR inhibitors
Applications
Isolated total RNA can be used in:
- RT-PCR
- RT-qPCR
- Northern Blotting
Includes
- Lysis Buffer (R2841)
- Wash Buffer 1 (conc.)(R2851)
- Wash Buffer 2 (conc.)(R2861)
- DNase l (lyophilized)
- DNase I Reconstitution Buffer
- 2x DNase I Buffer
- Manganese Chloride Solution
- Water, nuclease-free
- GeneJET RNA Purification Columns pre-assembled with Collection Tubes
- Collection Tubes, 2 mL
- Collection Tubes, 1.5 mL
Specifications
| 2X | |
| Spin Column | |
| 30 min. | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| 50 preps |
| For better long-term performance, it is recommended to store the purification columns at 2°C-8°C. | |
| Spin Column | |
| Blood | |
| Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction | |
| GeneJET | |
| Total RNA |
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Product Title
Thermo Scientific™ GeneJET Stabilized and Fresh Whole Blood RNA Kit > Quantity: 50 Preps
For Research Use Only. Not for use in diagnostic procedures.
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