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Thermo Scientific™ GeneBLAzer™ RAR-beta DA Assay Kit
GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Brand: Thermo Scientific™ K1407
This item is not returnable.
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Description
GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
Specifications
| Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid | |
| Cell-based beta lactamase reporter gene | |
| Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate: • RAR beta HEK 293T DA cells (K1407A) • LiveBLAzer™-FRET B/G Loading Kit, 70 μg Shipping: Dry Ice Storage: -80°C (liquid nitrogen) |
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| Fluorescence | |
| RARβ | |
| 9-cis-retinoic acid | |
| BLA (Beta-Lactamase) | |
| RAR beta, NR1B2, RARB | |
| 293T (HEK) | |
| Nuclear Receptors |
| none | |
| Division-Arrested Cells | |
| GeneBLAzer™ RAR-beta DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| Pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based Adme/tox assays, cellular adme assays, nuclear receptor cellular adme assays | |
| RAR beta HEK 293T DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg | |
| 1 plate | |
| 293T (HEK) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.