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Thermo Scientific™ GeneBLAzer™ NPSR1-A CHO-K1 DA Assay Kit
The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1493
This item is not returnable.
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Description
The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the nuclear factor of activated T-cell (NFAT) response element.
The NPSR1-A CHO-K1 DA cells and NPSR1-A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using Neuropeptide S. In addition, NPSR1-A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NP_997055.1 | |
| Division-Arrested Cells | |
| Fluorescence | |
| NPSR1-A | |
| Single Cell Clone Origin | |
| 1 Plate | |
| NPSR1-A | |
| CHO-K1 | |
| GPCR |
| Cell-based beta lactamase reporter gene | |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ NPSR1-A CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ NPSR1-A CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ NPSR1-A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| GPCRs | |
| GeneBLAzer™ NPSR1-A CHO-K1 DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70 μg, Solution D, GeneBLAzer™ NPSR1-A CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.