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Thermo Scientific™ FL - TRAP220DRIP1 PEP, 100 μM
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.
Brand: Thermo Scientific™ PV4598
Description
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.
How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).
Contents and Storage:
LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers. Store peptides at -20°C.
Specifications
≥90% by HPLC | |
100 μm | |
Fluorescein-TRAP220DRIP1 PEP, 100μL, 100μM Concentration, 2299Da Molecular Weight, –5 to –30°C Storage Condition, Used for Pharma and Biopharma, Target and Lead Identification and Validation | |
Classic Dyes | |
Fluorescein - TRAP220DRIP1 Peptide | |
For research use only. Not for use in diagnostic procedures | |
Dry Ice |
Fluorescein - TRAP220DRIP1 Peptide | |
Store in freezer (-5°C to -30°C). | |
Pharma and biopharma, target and lead identification and validation, drug discovery and development, nuclear receptor biology, nuclear receptor proteins, biochemical nuclear receptor assays, nuclear receptor coregulator interaction | |
2299Da | |
100 μL | |
Fluorescein-KVSQNPILTSLLQITGNGG | |
100% DMSO |
For Research Use Only. Not for use in diagnostic procedures.