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Thermo Scientific™ Pfu DNA Polymerase
Optimize PCR applications requiring high fidelity with this highly thermostable DNA polymerase.
Brand: Thermo Scientific™ EP0572
This item is not returnable.
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Description
Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction. Pfu DNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.
Highlights
- Eight times more accurate than Taq DNA polymerase
- Highly thermostable – remains 95% active after 2 hours incubation at 95°C
- Generates blunt-end PCR products
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
- Increased PCR yields with native Pfu DNA Polymerase
Specifications
| Pfu DNA Polymerase | |
| High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis | |
| 2 x 1.25mL 10X Pfu Buffer with (NH4)2SO2, 2 x 1.25mL 10X Pfu Buffer with MgSO4 and 2 x 1.25mL 25mM MgSO4 |
| 500U | |
| remains 95% active after 2 hours incubation at 95°C |