Learn More
Thermo Scientific™ DTT (dithiothreitol)
Shop All Thermo Scientific ProductsDescription
DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state .
At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.
- Free of endo-, exodeoxyribonucleases, ribonucleases and phosphatases
Recommended for:
Enzyme stabilization; Enzymatic reactions; Protein electrophoresis.
Note:
DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1M DTT solution, dissolve 1.55g of DTT powder in 10mL of deionized water (e.g., Water, nuclease-free).
Spécification
Spécification
| Concentration | 0.1 M |
| Product Type | DTT (Dithiothreitol) |
| Quantity | 5 g |
Sécurité et traitement
- DTT
- Warning
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P264-Wash thoroughly after handling.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
- MIXTURE LIST-Contains : 2, 3-Butanediol, 1,4-dimercapto-, (R*,R*)-
For Research Use Only. Not for use in diagnostic procedures.
Veuillez fournir vos retours sur le contenu du produit en remplissant le formulaire ci-dessous.